HEFOT HEPATOPROTECTIVE EFFECT OF FLAXSEEDS (LINUM USITATISSIMUM L.) ON TRIAZOPHOS INDUCED STEATOSIS AND HEPATIC ENZYMES IN MALE WISTAR RATS.
Abstract
Background: Inappropriate handling and use of pesticides, i.e. triazophos without any plan in agriculture have caused hepatotoxicity. The need for pesticide products for agricultural efficiency is clear, but the volume of production indicates that the occurrence of accidental exposure and as a result life threatening incidents are great.
Objective: To determine the effects of Flaxseeds on hepatic enzymes and steatosis in hepatocytes induced by Triazophos pesticide.
Methods: In this study 24 Wistar albino rats of age: 6-8 weeks and weight 150-200gms were segregated into four groups comprising six rats each (Random number Generator). Group A was labelled as control group. Groups B, C and D were labelled as experimental. All groups were given standard rat feed and water ad libitum. Group B was given powdered flaxseeds (800mg/kg b.w.) once daily for 21 consecutive days, Group C was given triazophos (8.2mg/kg b.w.) once daily for 21 consecutive days and Group D was given triazophos (8.2mg/kg b.w.) and flaxseeds (800mg/kg b.w.) once daily for 21 consecutive days. Livers of Albino rats were dissected out and their frozen sections were stained with Sudan ш to evaluate steatosis. Blood samples were collected from the tail of rats for biochemical evaluation of liver enzymes including alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP), which were assessed for biochemical changes on 1st ,7th,14th and 21st day. On day 22, 24 hours after administration of last dose, rats of control as well as of experimental groups were sacrificed under deep anesthesia.
Results: A statistically significant notable increase with a p value of 0.001, was observed in the hepatic enzymes and steatosis in livers of adult male albino rats. Additionally, Flaxseeds have significant ability to ameliorate the effects of Triazophos pesticide.
Conclusion: Use of Flaxseeds in our daily routine can decrease the drastic effects of pesticides on the liver.
