DETERMINATION OF HEPATIC HISTOTOPROTECTIVE EFFECT OF COMBINATION OF SM/ SILYMARIN AND ZNSO4/ ZINC SULFATE AGAINST DAMAGE BY RIF/ RIFAMPICIN AND INH/ ISONIAZID IN ALBINO RATS.

Abstract

Background: To induce hepatotoxicity with RIF and INH in albino rats, to estimate histoprotective effect of combination of ZnSO4 and Silymarin.

Objective: To evaluate the hepatic histoprotective activity of zinc sulfate in combination with silymarin against rifampicin- and isoniazid-induced hepatic damage in albino rats.

Methods: The study was conducted at the Department of Pharmacology, King Edward Medical University Lahore and UVAS, Lahore after approval from IRB committee vide no. 18/ PEC/RC/KEMU. The 'Animal Experimental Study' was carried out of 14 days’ duration. A sample size of 28 healthy albino wistar rats was taken and divided into four equal groups. GA was normal control group and given water once a day orally. GB was toxic control group and was given inducing agents RIF and INH (100 and 50 mg/kg/day) orally for 14 days. Whereas, rats in GC and GD received inducing agents along with combination of ZnSO4 + SM (3.5 + 100 mg /kg/day) and (7 + 200 mg /kg/day) respectively once daily for 14 days. Histoprotective effects evaluated by various histopathological parameters.

Results: Significant change (p-value 0.05) between positive control and hepatoprotective groups was observed for parameters like hepatic cell enlargement, lobular lymphocytic infiltrate, portal lymphocytic infiltrate, kuppfer cells, sinusoidal distention, lobular and portal plasma cellular infiltrate, steatosis, neutrophils, inflamed portal area, congestion and mast cells. Comparing GC and GD significant improvement was seen in parameters like lymphocytic lobular infiltrate, steatosis, hepatocytes enlargement and portal inflammation.

Conclusion: The current study has shown hepatoprotection of ZnSO4, SM and their combination in different doses as evidenced by improvement in histological parameters