CYCLOPHOSPHAMIDE RELATED HEPATOTOXICITY AND PROTECTION BY THYMOQUINONE, A HISTOLOGICAL VIEW

Abstract

Background: Cyclophosphamide (CP), used to cure cancer, causes hepatotoxicity. Hepatoprotection, brought by thymoquinone (TQ) found in kalonji. Study was performed to prove it.

Objectives: To observe thymoquinone restorative effect on the hepatic toxicity by cyclophosphamide.   

Methods:  It is Experimental randomized control trial. Adult albino rats were 24, put in 3 groups, each having 8. control Group A, given 2 ml Phosphate Buffer Saline (PBS) for a week by gastric gavage, 2 intraperitoneal injection (i.p) 2 days apart, gastric gavage for 1 week again. Experimental Group B, two i.p doses of CP as 200 mg/kg, on day 1, 4. Group C, given TQ (10 mg/kg/day) by gastric gavage for 1 week, 2 doses of CP, given on day 8 and 10, again TQ for 1 week. 24 hours after the experiment, livers were isolated from rats after dissection and processed. Gross and histopathological changes were observed.

Results: Weight of rats of both experimental groups (B, C) was decreased in comparison to A group (p-value <0.001), reduced from (190.4 g-136.9 g) for group B, (177 g-147g) for group C rats, weight increased in group A rats (184.9 g-223.1g). RTWI, more in both groups (4.38 in B and 3.55 in C) in comparison to control (2.59), B versus A (p-value <0.001), B versus C (p-value 0.012) and C versus A (p-value 0.004). Hepatic lobules shape difference was statistically significant in experimental groups when compared to the control (p-value <0.001).

Conclusion: Study proved hepatotoxic impact of CP on liver histology, shows ameliorative effect of TQ.