MATRIX METALLOPROTEINASE-2 GENE POLYMORPHISM AND SUSCEPTIBILITY TO MYOCARDIAL INFARCTION

Abstract

Background: Myocardial infarction (MI) is a major cause of death in Pakistan. In pathogenesis of MI, Matrix Metalloproteinases-2 (MMP-2) acts as a major contributor by causing extracellular matrix degradation and atherosclerotic plaque rupture. MMP-2 -1306 C/T polymorphism (rs243865) is a variation in MMP-2 gene that increase the risk of MI.

Objectives: The objective of this study was to find out the association of the MMP-2 -1306 C/T polymorphism with development of MI in the Pakistani population.

Materials: It was a case-control study with 36 acute MI patients and 36 matched healthy controls. MMP-2 gene was augmented by Polymerase Chain Reaction and genotype was found out by Restriction Fragment Length Polymorphism (PCR-RFLP). SPSS 26.0 was used to perform statistical analysis.

Results: The heterozygous CT genotype was significantly higher in MI cases (27.8%) than in controls (5.6%; p = 0.01), with an odds ratio of 6.53 (95% CI 1.3–32.4). The T allele was also significantly linked with  MI (p = 0.016). No significantly substantial association was determined between this polymorphism and traditional clinical risk factors, serum MMP-2 levels, or cardiac biomarkers (p > 0.05).

Conclusion: The CT genotype and T allele of the MMP-2 -1306 C/T polymorphism are significantly associated with development of MI. This polymorphism may act as an independent genetic risk marker in the Pakistani population.